Ion Transfer Through DPPC Monolayers

 

H.A. Santos^a, C. M. Pereira,* F. Silva

 

Departamento de Química, Faculdade de Ciências do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal.

^aPresent address: Laboratory of Physical Chemistry and Electrochemistry, Helsinki University of Technology, PO Box 6100, FIN-02015 HUT, Finland.

 

 

Abstract

The transfer of acetylcholine cation through a monolayer of a lipid, dipalmitoylphosphatidylcholine, formed at a polarized water | 1,2-dichloroethane interface has been studied using electrochemical impedance spectroscopy. The ion transfer process was characterized at different levels of interfacial coverage. From measurement of the cyclic voltammograms and double layer capacitance it was found that cation transfer through dipalmitoylphosphatidylcholine monolayers was cation and phospholipid concentration dependent. An increase on current intensity and capacitance values was observed at more positive potentials due to the transfer of acetylcholine across the interface in the absence or presence of lipid in the organic phase. The thermodynamic and kinetic parameters were also accessed from electrochemical impedance measurements.

 

Keywords: ion transfer, acetylcholine, monolayer, phospholipids, DPPC, impedance spectroscopy.

 

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* Corresponding author. E-mail address: cmpereir@fc.up.pt